You bring up several good points. We know that bacteria often act very differently inside the body (in vivo) then in the lab (ex vivo) so I don’t think we can ever be completely sure that what happens in the lab would also happen in the body. Still, it would seem to me that if someone with an e.coli infection were administered cefulodin they would certainly develop a plethora of e.coli L-forms. In my opinion doctors need to know this information ASAP.

I also considered the possibility that different beta-latams might work more or less effectively to transform different species of bacteria into the L-form. It’s very possible that different specific techniques will be created to culture different L-form species. There is so much research yet to be done in this field! I eagerly await a time when researchers working with L-form bacteria will be given the funding they deserve.

Best,

Amy

Very interesting article and it looks like an important breakthrough in the lab preparation of L-form CWD bacteria, at lest for E. coli.

As I understand it, it is difficult for penicillin to develop the L-form bacteria because all the penicillin binding proteins (PBP) are inactivated. However, when using the antibiotic cefulodin, the correct combination of PBC on/off is achieved for E. coli and the E. coli L-form are obtained “over-night”.

Then, does this mean that some antibiotics like cefulodin will cause rapid conversion to the L-form for those who take that abx for a particular disease? In that case, it seems that those people would be quickly infected with the L-form bacteria.

I wonder if this PBC on/off combination is unique to E. coli and that the correct combination must also be determined for each bacteria?

Gene

You are so supportive of everyone on the MP and make a big difference on the site by answering people’s questions. Thanks for passing on my work especially to a microbiologist who one would hope would be interested in learning more about L-forms. I really admire you positive attitude!

Amy

Good question.

I admit that the analysis of antibiotics and their ability to induce or not induce the formation of L-form bacteria in D’Ari’s article is quite complex.

Despite the fact that it blocks all the PBPs, penicillin can, and does cause the formation of L-form bacteria. The point that the authors were trying to make is that because it blocks all the PBPs, penicillin is quite bad at actually forming them. So it seems that even though it blocks all the PCPs transformation can occur, just through a much more lengthy process. In fact, the process takes years, rather than the one day it takes to culture L-forms using cefulodin.

The process by which L-forms are created using pencillin as decribed by D’Ari required researchers to “spread a heavy inoculum of bacteria on a plate of hypertonic complex medium containing serum, broth, and penicillin. After a growth period, usually of several weeks, an agar block is cut from the plate, inverted, and spread on a plate of the same medium for a new growth period. Such “passages” are repeated serially, often for years, until finally a stable L form appears, able to grow indefinitely and no longer able to revert to normal morphology when cultivated in the absence of penicillin.”

Yikes! That is a seriously long procedure. So the point is even with all the PBPs turned off L-form can be created, but keeping some of them on makes the process much more efficient.

However keeping all the PBPs ON is another story. Inactivation of the PBPs is what allows the wall of a classical bacterial form to lose it’s ability to stay rigid and complete. When all the PBPs are on, the cell wall is quite sturdy and the cell simply can’t begin the process of transforming into the L-form which, as we know, retains little of the cell wall.

Hope this helps!

Amy

This is a fascinating article.

It seems strange to me that you would be looking for something that would not block any of the PBPs, as blocking them is how Penicillin functions at all.

Do you mean that we need another drug that would somehow knock off the bacteria without attacking the PBPs?

Best,

Cass A

I’m sorry to hear that your doctor is so unwilling to let you start the Marshall Protocol. Every doctor should know that you can test the level of the two vitamin D metabolites in the body, so if your doctor doesn’t even realize that, I suggest that you find another doctor. You’re right. It’s important to start the treatment as soon as possible, and it’s also very important to work with a physician who is at least supportive.

What I recommend doing is requesting a list of doctors in your area that are willing to put patients on the Marshall Protocol and are already familiar with the treatment. You can do so at this link:

http://www.marshallprotocol.com/forum11/9355.html

If it comes down to it, you could even fly to see a competent MP doctor in another location and arrange a way for follow-up visits to take place over the phone. Other people on the MP have done that successfully.

I also recommend posting on the following website:

http://www.curemyth1.org (Th1 refers to diseases caused by L-form bacteria, hence the name Cure My Th1). The patient advocates on that site will answer your questions free of charge. They help people find doctors all the time and may be able to offer further advice.

If you must stick with your current doctor for insurance reasons, the patient advocates at curemyth1 can show you where to find articles about the MP and Dr. Marshall’s papers. It might be helpful to present these documents to your doctor in order to help hin better understand the treatment.

Good luck!

Amy

Great work, Amy.